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113 results
2021
Witvliet, D. et al. Connectomes across development reveal principles of brain maturation. Nature 596, 257–261 (2021).
Witvliet, D. et al. Connectomes across development reveal principles of brain maturation. Nature 596, 257–261 (2021).
From birth to adulthood, an animal’s nervous system changes as its body grows and its behaviours mature. The form and extent of circuit remodelling across the connectome is unknown. We used serial-section electron microscopy to reconstruct the full brain...
2020
Nejatbakhsh, A. et al. Extracting neural signals from semi-immobilized animals with deformable non-negative matrix factorization. bioRxiv (2020).
Nejatbakhsh, A. et al. Extracting neural signals from semi-immobilized animals with deformable non-negative matrix factorization. bioRxiv (2020).
Extracting calcium traces from populations of neurons is a critical step in the study of the large-scale neural dynamics that govern behavior. Accurate activity extraction requires the correction of motion and movement-induced deformations as well as...
Choi, J. et al. Probing and manipulating embryogenesis via nanoscale thermometry and temperature control. Proceedings of the National Academy of Sciences 117, 14636–14641 (2020).
Choi, J. et al. Probing and manipulating embryogenesis via nanoscale thermometry and temperature control. Proceedings of the National Academy of Sciences 117, 14636–14641 (2020).
Temperature is a key control parameter of biological processes, but measuring and controlling temperatures on a cellular-length scale in living organisms remains an outstanding challenge. Applying nanoscale-thermometry techniques to early embryos, we...
Yemini, E. et al. NeuroPAL: A Neuronal Polychromatic Atlas of Landmarks for Whole-Brain Imaging in C. elegans. Cell 184, 272–288 (2020).
Yemini, E. et al. NeuroPAL: A Neuronal Polychromatic Atlas of Landmarks for Whole-Brain Imaging in C. elegans. Cell 184, 272–288 (2020).
Comprehensively resolving single neurons and their cellular identities from whole-brain fluorescent images is a major challenge. We achieve this in C. elegans through the engineering and use of a multicolor transgene called NeuroPAL (a Neuronal Polychroma...
Mi, L. et al. Learning Guided Electron Microscopy with Active Acquisition. in Medical Image Computing and Computer Assisted Intervention – MICCAI 2020 77–87 (Springer International Publishing, 2020).
Mi, L. et al. Learning Guided Electron Microscopy with Active Acquisition. in Medical Image Computing and Computer Assisted Intervention – MICCAI 2020 77–87 (Springer International Publishing, 2020).
Single-beam scanning electron microscopes (SEM) are widely used to acquire massive datasets for biomedical study, material analysis, and fabrication inspection. Datasets are typically acquired with uniform acquisition: applying the electron beam with the...
Nejatbakhsh, A. et al. Demixing Calcium Imaging Data in C. elegans via Deformable Non-negative Matrix Factorization. in Medical Image Computing and Computer Assisted Intervention – MICCAI 2020 14–24 (Springer International Publishing, 2020).
Nejatbakhsh, A. et al. Demixing Calcium Imaging Data in C. elegans via Deformable Non-negative Matrix Factorization. in Medical Image Computing and Computer Assisted Intervention – MICCAI 2020 14–24 (Springer International Publishing, 2020).
Extracting calcium traces from the neurons of C. elegans is an important problem, enabling the study of individual neuronal activity and the large-scale dynamics that govern behavior. Traditionally, non-negative matrix factorization (NMF) methods have...
2019
Budelli, G. et al. Ionotropic Receptors Specify the Morphogenesis of Phasic Sensors Controlling Rapid Thermal Preference in Drosophila. Neuron 101, 738–747 (2019).
Budelli, G. et al. Ionotropic Receptors Specify the Morphogenesis of Phasic Sensors Controlling Rapid Thermal Preference in Drosophila. Neuron 101, 738–747 (2019).
Thermosensation is critical for avoiding thermal extremes and regulating body temperature. While thermosensors activated by noxious temperatures respond to hot or cold, many innocuous thermosensors exhibit robust baseline activity and lack discrete...
Si, G. et al. Structured Odorant Response Patterns across a Complete Olfactory Receptor Neuron Population. Neuron 101, 950–962 (2019).
Si, G. et al. Structured Odorant Response Patterns across a Complete Olfactory Receptor Neuron Population. Neuron 101, 950–962 (2019).
Summary Odor perception allows animals to distinguish odors, recognize the same odor across concentrations, and determine concentration changes. How the activity patterns of primary olfactory receptor neurons (ORNs), at the individual and population...
Calarco, J. A. & Samuel, A. D. T. Imaging whole nervous systems: insights into behavior from brains inside bodies. Nature Methods 16, 14–15 (2019).
Calarco, J. A. & Samuel, A. D. T. Imaging whole nervous systems: insights into behavior from brains inside bodies. Nature Methods 16, 14–15 (2019).
The development of systems combining rapid volumetric imaging with three-dimensional tracking has enabled the measurement of brain-wide dynamics in freely behaving animals such as worms, flies, and fish. These advances provide an exciting opportunity to...
2018
He, L., Si, G., Huang, J., Samuel, A. D. T. & Perrimon, N. Mechanical regulation of stem-cell differentiation by the stretch-activated Piezo channel. Nature 555, 103–106 (2018).
He, L., Si, G., Huang, J., Samuel, A. D. T. & Perrimon, N. Mechanical regulation of stem-cell differentiation by the stretch-activated Piezo channel. Nature 555, 103–106 (2018).
Stem cells of the Drosophila midgut sense mechanical signals in vivo through the stretch-activated ion channel Piezo, which is expressed on previously unidentified enteroendocrine precursor cells.
Hawk, J. D. et al. Integration of plasticity mechanisms within a single sensory neuron of C. elegans actuates a memory. Neuron 97, 356–367 (2018).
Hawk, J. D. et al. Integration of plasticity mechanisms within a single sensory neuron of C. elegans actuates a memory. Neuron 97, 356–367 (2018).
Neural plasticity, the ability of a neuron to change its cellular properties in response to past experiences, underpins the nervous system’s capacity to form memories and actuate behaviors. How different plasticity mechanisms act together in vivo and at a...
Humberg, T.-H. et al. Dedicated photoreceptor pathways in Drosophila larvae mediate navigation by processing either spatial or temporal cues. Nature Communications 9, 1260 (2018).
Humberg, T.-H. et al. Dedicated photoreceptor pathways in Drosophila larvae mediate navigation by processing either spatial or temporal cues. Nature Communications 9, 1260 (2018).
To integrate changing environmental cues with high spatial and temporal resolution is critical for animals to orient themselves. Drosophila larvae show an effective motor program to navigate away from light sources. How the larval visual circuit processes...